Structures of Large RNA Molecules and Their Complexes

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs. Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes INDICE: I. RNA Folding and Dynamics 1. RNA dynamics by NMR   Hashim M. Al-Hashimi 2. Helix dynamics by Au-nanoparticle SAXS   Daniel Herschlag and Pehr A. B. Harbury 3. Spectroscopic probes for RNA dynamics   Kathleen B. Hall 4. Analysis of sequencing based RNA structure probing data   Joel McManus 5. In vivo hydroxyl radical footprinting of rRNA   Jeppe Vinther 6. Model of p65 telomerase complex by SAXS   Robert Paul Rambo 7. Computational tools for RNA structure validation and improvement   Jane S. Richardson 8. Native purification and analysis of long RNAs   Anna Marie Pyle 9. Structural biology of riboswitches   Ailong Ke II. RNA-protein interactions 10. RRM-RNA interactions   Frederic Allain 11. Use of EPR in RNA and protein-RNA complexes   Gunnar Jeschke 12. Use of paramagnetic relaxation enhancement in protein-RNA complex   Michael Sattler 13. SAXS and SANS to investigate protein-RNA complexes   Frank Gabel 14. Mass spectrometric analysis of RNA-protein interactions   Henning Urlaub 15. NGS and RNA binding affinity   Chris Burge III. Large RNPs 16. MD methods for analysis of cryo-EM structures   Karissa Sanbonmatsu 17. CRISPR complexes   Martin Jinek 18. Single-Molecule Pulldown FRET (SiMPull-FRET) to dissect biomolecular machines   Nils G. Walter 19. Live-Cell Visualization of Pre-mRNA Splicing   Maria Carmo-Fonseca

• ISBN: 978-0-12-801934-4
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/06/2015
• Nº Volúmenes: 1
• Idioma: Inglés