Quantitative Imaging in Cell Biology: Methods in Cell Biology

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications.  The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material INDICE: 1. Concepts in Quantitative Fluorescence Microscopy2. Practical Considerations of Objective Lenses for Application in Cell Biology3. Assessing Camera Performance for Quantitative Microscopy4. A Practical Guide to Microscope Care and Maintenance5. Fluorescence Live Cell Imaging6. Fluorescent Proteins for Quantitative Microscopy: Important Properties and Practical Evaluation7. Quantitative Confocal Microscopy: Beyond a Pretty Picture8. Assessing and Benchmarking Multiphoton Microscopes for Biologists9. Spinning Disk Confocal Microscopy: Present Technology and Future Trends10. Quantitative Deconvolution Microscopy11. Light Sheet Microscopy12. DNA Curtains: Novel Tools for Imaging Protein-nucleic Acid Interactions at the Single-molecule Level13. Nanoscale Cellular Imaging with Scanning Angle Interference Microscopy14. Localization Microscopy in Yeast15. Imaging Cellular Ultrastructure by PALM, iPALM, and Correlative iPALM-EM  16. Seeing more with Structured Illumination Microscopy17. Structured Illumination Super-Resolution Imaging of the Cytoskeleton18. Analysis of Focal Adhesion Turnover: A Quantitative Live Cell Imaging Example19. Determining Absolute Protein Numbers by Quantitative Fluorescence Microscopy 20. High Resolution Traction Force Microscopy21. Experimenters' Guide to Co-Localization Studies: Finding a Way Through Indicators and Quantifiers, in Practice22. User-Friendly Tools for Quantifying the Dynamics of Cellular Morphology and Intracellular Protein Clusters23. Ratiometric Imaging of pH Probes24. Towards Quantitative Fluorescence Microscopy with DNA Origami Nanorulers25. Imaging and Physically Probing Kinetochores in Live Dividing Cells26. Adaptive Fluorescence Microscopy by Online Feedback Image Analysis 27. Open Source Solutions for SPIMage Processing28. Second Harmonic Generation Imaging of Cancer

• ISBN: 978-0-12-420138-5
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 464
• Fecha Publicación: 01/07/2014
• Nº Volúmenes: 1
• Idioma: Inglés