This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on DNA sensors and inflammasomes INDICE: 1. Phosphorylation and dimerization of STING and IRF3 Søren R. Paludan 2. cGAS enzymology Philip J. Kranzusch 3. Synthesis and identification of immuno-stimulatory CDNs Jörg S. Hartig 4. Tracking cGAS activity/ cGAMP formation using SPR/NMR Erik C. Ralph 5. Using an enzyme coupled assay to track cGAS activity under steady states Jungsan Sohn 6. Tracking the polymerization of DNA sensors, inflammasome receptors, and downstream signaling partners using FRET Jungsan Sohn 7. NLRC4 structure Hao Wu 8. Tracking TREX1 activity Fred W. Perrino 9. DNA association and dissociation kinetics of PARP1 Johannes Rudolph 10. Using AFM to track DNA: sensor complex formation Alexey Krasnoslobodtsev 11. Using electron microscopy methods for visualizing DNA: sensor and inflammasome complexes (prep for cryo-EM) Jungsan Sohn 12. Methods for cryo-EM sample prep/ reconstruction of the NAIP.NLRC4 complex Eva Nogales 13. Innovative methods that underlie the solid state NMR studies of the inflammasome Sebastian Hiller 14. Using NMR for probing ASC-ASC interaction Eva de Alba 15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy Brendan Antiochos 16. Monitoring IL-1B and IFN-1 output levels NLRP3 and cGAS agonists Michael A. Chattergoon 17. ATM and inflammasome assembly Nelson O. Gekara 18. STING/TBK Leonie Unterholzner 19. cGAS and mitotic cell death Nelson O. Gekara 20. Tracking PTMs of DNA sensors Ileana M. Cristea 21. Ubiquitination Nelson O. Gekara
- ISBN: 978-0-12-818359-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/09/2019
- Nº Volúmenes: 1
- Idioma: Inglés