Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In EnzymologyContains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization INDICE: I - Recombinant Expression 1. Cell-Free expression of membrane proteins Frank Bernhard 2. Expression of recombinant GPCRs in E. coli Anne Skaja Robinson 3. Recombinant expression of a plant ion channel VDAC4 M K. Mathew 4. Expression of membrane proteins in Wolinella Roy Lancaster 5. Expression of recombinant membrane proteins in Lactococcus lactis Edmund Kunji 6. Heterologous Expression of GPCRs in Yeast Bernadette Byrne 7. Expression of recombinant membrane proteins in Yeast Nico Callewaert and Katrien Claes 8. Expression of non-selective Ion channel TRPM4 in Yeast Benjamin Clémençon 9. Baculovirus mediated expression of GPCRs in insect cells Adrian Goldman, Tuulia Saarenpää and Veli-Pekka Jaakola 10. ACEMBLing a Multiprotein Transmembrane Complex: The Functional SecYEG-SecDFYajC-YidC Holotranslocon Protein Secretase/Insertase Imre Berger 11. Drosophila photoreceptor cells for recombinant expression of membrane proteins Irmgard Sinning 12. Expression of GPCRs in mammalian cells Prashen Chelikani 13. Tetracycline inducible system for expression of recombinant membrane proteins Philip J. Reeves 14. Semliki Forest Virus based expression of recombinant GPCRs Kenneth Lundstrom II - Functional Characterization of Recombinant Membrane Proteins 15. Lipid reconstitution and transport assay for recombinant transporters Seok-Yong Lee 16. Ion channel reporter for monitoring the activity of engineered GPCRs Christophe J. Moreau 17. Reconstitution and current recording of recombinant ion channels in liposomes Ming-Feng Tsai 18. Expression and functional characterization of a Nitrate Transporter, NirC Oliver Einsle and Adriana Rycovska-Blume 19. Incorporation of Membrane proteins in Nanodiscs for functional studies Anthony Watts 20. Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis James Fessenden 21. Directed cross-linking strategy for functional characterization of membrane proteins Jeffrey Becker 22. Interaction of Rhodopsin with G protein and arrestin Klaus Peter Hofmann 23. Surface Plasmon Resonance for investigating Ligand-Receptor interactions Iva Hopkins 24. Fluorescence approaches to study oligomerization of membrane proteins Amitabha Chattopadhyay 25. Rhodopsin expression in HEK cells for SDSL/EPR studies Oliver P. Ernst 26. Engineering Escherichia coli for functional expression of membrane proteins Bert Poolman and Franz Yiu-Fung Ho 27. Biophysical studies on Beta2 adrenergic receptor and its signaling complexes Arun K. Shukla
- ISBN: 978-0-12-801521-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 01/04/2015
- Nº Volúmenes: 1
- Idioma: Inglés