Membrane proteins - Engineering, Purification and Crystallization

Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In EnzymologyContains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization INDICE: I - Membrane protein engineering 1. Fusion approaches in GPCR crystallization   Arun K. Shukla 2. A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs   Mark E. Dumont 3. Fluorescence based screening for purification of membrane protein complexes   Robert Tampé II - Solubilization and purification of membrane proteins 4. A general protocol for membrane protein solubilization   Ankita Roy 5. Novel classes of amphiphiles and detergents for membrane protein purification   Pil Seok Chae 6. Quantification of detergents in purified membrane protein samples   Zongchao Jia 7. Purification of a sodium/proton transporter   Etana Padan 8. Purification of an outer membrane protein OmpG   Özkan Yildiz 9. Purification of a lecuine transporter LeuT   Satinder K. Kaur Singh III -Biophysical studies of membrane proteins 10. Structural characterization of membrane proteins by Electron Microscopy   Ingeborg Schmidt-Krey 11. EPR studies on a voltage gated potassium channel   Sudha Chakarpani 12. Structure and dynamics of membrane proteins by NMR spectroscopy   Marc Baldus 13. Hydrogen-Deuterium Exchange of a GPCR-G protein signaling complex   Ka Young Chung 14. Electron Tomography of Membrane Proteins   Ohad Medalia 15. Spectroscopic characterization of recombinant membrane proteins   Linda Columbus III - Computational Approaches to Membrane Proteins 16. Computational approaches to understand the aquaporin Channels   Ramasubbu Sankararamakrishnan 17. Mix and Match? approach to understand MFS transporters   M. Gregor Madej 18. Molecular dynamics simulation of GPCR activation in lipid bilayer   Diwakar Shukla and Morgan Lawrenz IV - Crystallization of Membrane Proteins 19. Antibody fragment assisted crystallization of membrane proteins   Carola Hunte 20. Synthetic antibodies as crystallization chaperones for membrane proteins   Anthony Kossiakoff 21. Crystallization of membrane proteins by vapor diffusion   Edward Yu 22. Crystallization of membrane proteins in bicelles   Salem Faham 23. LCP-FRAP assay for pre-crystallization screening of membrane proteins   Vadim Cherezov 24. Crystallization of native membrane proteins from bovine rod outer segment   Krzysztof Palczewski

• ISBN: 978-0-12-802183-5
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/05/2015
• Nº Volúmenes: 1
• Idioma: Inglés