Biological Identification: DNA Amplification and Sequencing, Optical Sensing, Lab-On-Chip and Portable Systems

Biological identification systems are a vital component in countering the threats to life and health posed by natural pathogens, toxins, and bioterrorism agents. It is essential that such systems are fast, accurate, reliable and easy to use. It is also important to employ the most suitable technology in dealing with any particular threat. This book provides a detailed review of the technologies available and the potential for future developments. Part one covers the essentials of DNA and RNA sequencing for the identification of pathogens, including next generation sequencing (NGS), polymerase chain reaction (PCR) methods, isothermal amplification and bead array technologies. Part two addresses a variety of approaches to making identification systems portable, tackling the special requirements of smaller, mobile systems in fluid movement, power usage, and sample preparation. Part three focuses in on a range of optical methods and their advantages. Finally, part four describes a unique approach to sample preparation and a promising approach to identification using mass spectroscopy. Biological Identification will be a useful resource for academics and engineers involved in the microelectronics and sensors industry, and for companies, medical organisations and military bodies looking for biodetection solutions. Covers DNA sequencing of pathogens, lab-on-chip and portable systems for biodetection and analysisProvides an in-depth description of optical systems and explores sample preparation and mass spectrometry-based biological analysis INDICE: Part 1 Technology for DNA and RNA analysis of pathogens Nucleic acid sequencing for characterizing infectious and/or novel agents in complex samples C. Detter, Los Alamos National laboratory, USA Multiplexed, lateral flow, polymerase chain reaction (PCR) techniques for biological identification W. M. Nelson, G. W. Long and L. M. Cockrell, Tetracore, USA Isothermal amplification of specific sequences Y. Tong, New England Biolabs, USA Bead array technologies for genetic disease screening and microbial detection S.A. Dunbar and S.V. Angeloni, Luminex Corporation, USA Part 2 Lab-on-chip and portable systems for biodetection and analysis Electrochemical detection for biological identification P. Skládal, Masaryk University, Czech Republic Conductometric biosensors S. Dzyadevych, National Academy of Sciences of Ukraine, Ukraine and N. Jaffrezic-Renault, University of Lyon, France Bio-chem-FETs: field effect transistors for biological sensing N. Chaniotakis and M. Fouskaki, University of Crete, Greece Microfluidic devices for rapid identification and characterization of pathogens H. Becker, Microfluidic ChipShop GmbH, Germany Part 3 Optical systems for biological identification Optical Biodetection using Receptors and Enzymes (Porphyrin-Incorporated) H. J. Harmon and A. Oliver, Oklahoma State University, USA Overview of THz spectral characterization for biological identification T. Globus, B. Gelmont and I. Sizov, University of Virginia, USA Raman spectroscopy for biological identification T.J. Ronningen, J.M. Schuetter, J.L. Wightman, A. Murdock and A.P. Bartko, Battelle Memorial Institute, USA LIDAR (light detection and ranging) for biodetection H.S. Lee, C. R. Prasad, and R. M. Serino, Science & Engineering Services, Inc. (SESI), USA Part 4 Sample preparation and mass spectrometry-based biological analysis Electrophoretic approaches to sample collection and preparation for nucleic acids analysis. C. Bradburne, Johns Hopkins University, USA Mass spectrometry-based proteomics techniques for biological identification R.E. Jabbour and A. P. Snyder, US Army Edgewood Chemical Biological Center, USA

• ISBN: 978-0-85709-501-5
• Editorial: Woodhead Publishing
• Encuadernacion: Cartoné
• Páginas: 390
• Fecha Publicación: 23/05/2014
• Nº Volúmenes: 1
• Idioma: Inglés