Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatialresolution and minimized sample charging and damage. Images produced are lessdestroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limitedto journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples.It provides a comprehensive explanation of insturmentation, applications, andprotocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images. INDICE: Historical Preface The early Devevlopment of the Scanning ElectronMicroscope to 1965. LVSEM For Biology. The Aberration-Corrected SEM. Noise and its effects on the Kow Voltage SEM. High -Resolution Low Voltage Field-Emission Scanning Electron Microscopy Applications for Cell Biology and Specimen Preparation Protocols.-Molecular labeling for Correlative Microscopy: LM, LVSEM,TEM, EF-TEM and HVEM.-Low kV and video-rate, beam-tilt stereo for viewing live-time experiments in the SEM. CryoSEM of Chemically Fixed Animal Cells. High resolution and low voltage SEM of plant cells. High Resolution Cryo-Scanning Electron Microscopy of Biological Samples. Developments in Instrumentation for Microanalysis in Low Voltage Scanning Electron Microscopy.
- ISBN: 978-0-387-72970-1
- Editorial: Springer
- Encuadernacion: Cartoné
- Páginas: 318
- Fecha Publicación: 01/01/2008
- Nº Volúmenes: 1
- Idioma: Inglés