Terpene Synthases, Volume 700 in the Methods in Enzymology series, continues the legacy of this highly respected laboratory standard with its first dedicated collection on this important family of enzymes. Terpene synthases are a diverse set of enzymes that use exquisite mechanisms to form complex (poly)cyclic hydrocarbon skeletons. Chapters in this new volume include Structural analysis by X-ray crystallography and cryo-EM, Understanding mechanisms using stable isotopes, substrate analogs, or computational tools, Engineering fusion enzymes, Ancestral terpene cyclases, as well as the Sequence, structure, and function of non-canonical terpene synthases. Presents the only collection of current methodology for the investigation of terpene synthases, with topics including from bioinformatics, enzymology, computational chemistry, and engineeringIncludes chapters authored by international experts in the fieldProvides the latest contributions in the leading serial Methods in Enzymology INDICE: 1. Methods for the Expression, Purification, and Structural Analysis of a Bifunctional Class I Diterpene Synthase, Fusicoccadiene Synthase2. Methods for the Expression, Purification, and Structural Analysis of a Bifunctional Class II Diterpene Synthase, Copalyl Diphosphate Synthase3. X-ray Crystallography of Terpene Synthases4. Isotopic Labellings for Mechanistic Studies5. Understanding Mechanisms of Terpene Synthases Using Substrate Analogs6. Docking Carbocations into Terpene Synthase Active Sites using Chemically Meaningful Constraints - The Terdockin Approach7. A Practical Tutorial/Guide to EnzyDock8. Bioinformatics of Terpene Synthases9. Ancestral Terpene Cyclases: From Fundamental Science to Applications in Biosynthesis10. Coral Terpene Synthases11. Deciphering Magnesium Binding Site and Structure-Function Insights in Class II Sesquiterpene Cyclase12. Functional Characterization of the UbiA-Family of Terpene Synthases13. Functional Analysis of Transmembrane Terpene Cyclases Involved in Fungal Meroterpenoid Biosynthesis14. Vanadium Haloperoxidases as Noncanonical Terpene Synthases15. Structure, Function, and Engineering of Large Terpene Synthases16. Coral Terpene Synthases (and perhaps some genomics and genome mining)17. Engineering Terpene Synthases via Substrate Analogs18. Engineering Terpene Synthase Fusions Enzymes
- ISBN: 978-0-443-23808-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 536
- Fecha Publicación: 01/07/2024
- Nº Volúmenes: 1
- Idioma: Inglés