G4 Biology, Volume 695 in the Methods in Enzymology series, highlights high value topics such as Assays to measure G4 resolution by DNA helicases, In gel staining methods of G4 DNA and RNA structures, Methods to probe the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolution, Analyzing the effect of transcription coupled R-loop formation on G4 formation, Integrated Structural Biology for the study of higher-order G4 structures, Advancing the Study of Protein-G4 Interactions in DNA Repair: Insights from Biolayer Interferometry and Circular Dichroism Spectroscopy, Immunofluorescence microscopy (IF) of G-quadruplex and R-loops, and more. Additional chapters delve into Production of the anti-G-quadruplex antibody BG4 for efficient ChIP-seq and CUT&Tag analysis: from plasmid quality control to validation, Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures, Potentiometric titrations to study ligand interactions with DNA i-motifs, Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR Spectroscopy, and Identification of bona fide G4-binding proteins, Observing Pif1 opening dsDNA and G4 in real time by manipulation under magnetic tweezers, and in vivo detection of DNA secondary structures using Permanganate/S1 Footprinting with direct adapter ligation and sequencing (PDAL-Seq). Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in Methods in Enzymology seriesUpdated release includes the latest information on G4 biology INDICE: 1. Assays to measure G4 resolution by DNA helicasesRobert Brosh2. In gel staining methods of G4 DNA and RNA structuresKatrin Paeschke3. Methods to probe the folding pathways of four-stranded intercalated cytosine-rich motifs at single base-pair resolutionNasim Sabouri4. Analyzing the effect of transcription coupled R-loop formation on G4 formationSua Myong5. Integrated Structural Biology for the study of higher-order G4 structuresJonathan Chaires6. Advancing the Study of Protein-G4 Interactions in DNA Repair: Insights from Biolayer Interferometry and Circular Dichroism SpectroscopyColin Wu7. Immunofluorescence microscopy (IF) of G-quadruplex and R-loopsGiovanni Capranico8. Observing Pif1 opening dsDNA and G4 in real time by manipulation under magnetic tweezersJean-Baptiste Boulé9. in vivo detection of DNA secondary structures using Permanganate/S1 Footprinting with direct adapter ligation and sequencing (PDAL-Seq)Angelika Lahnsteiner10. Production of the anti-G-quadruplex antibody BG4 for efficient ChIP-seq and CUT&Tag analysis: from plasmid quality control to validationSara Richter11. Utility of intercalator displacement assays for screening of ligands for i-motif DNA structuresZoë Waller and Dilek Guneri12. Potentiometric titrations to study ligand interactions with DNA i-motifsAnton Granzhan13. Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR SpectroscopyLukás Trantírek14. Identification of bona fide G4-binding proteinsPavel Ivanov
- ISBN: 978-0-443-21774-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 296
- Fecha Publicación: 21/03/2024
- Nº Volúmenes: 1
- Idioma: