This new volume of Methods in Cell Biology looks at methods for lysosomes and lysosomal diseases. Chapters focus upon practical experimental protocols to guide researchers through the analysis of multiple aspects of lysosome biology and function. In addition, it details protocols relevant to clinical monitoring of patients with lysosomal diseases. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studiesChapters are written by experts in the fieldCutting-edge material INDICE: 1. Biosynthesis, targeting and processing of lysosomal proteins: pulse-chase labeling Sandra Pohl and Andrej Hasilik 2. Lysosome subcompartments3. Measuring lysosomal storage and its consequences in the CNS Jon Cooper4. Imaging approaches to measuring Ca++ release. Ant Morgan, Lianne Caroline Davis and Antony Galione 5. Elecrophysiology Xianping Dong6. Single-channel recordings in artificial membranes Rebecca Sitsapesan and Elisa Venturi 7. Morphometric analysis Sandip Patel and Clare Futter 8. Autophagy Barry Boland9. Lysosomal pH Sergio Grinstein10. TFEB/ CLEAR Network Andrea Ballabio, Diego Medina and Carmine Settembre 11. Lysosome fusion John Paul Luzio12. Phagocytosis Nick Platt13. Microbial manipulation of lysosome maturation Maria Podinovskaia14. Measuring relative lysosomal volume for monitoring lysosomal storage diseases Danielle te Vruchte and Fran Platt 15. Clinical diagnosis of NPC disease: the filipin staining method Marie Vanier and Philippe Latour 16. Lysosomal interactions with ER Colin Taylor17. RNAi Screens of lysosomal trafficking Michael Barry Brenner and Salil Garg 18. Lysosomal Cell Death Marja Jaattella, Sonja Aits and Jesper Nylandsted 19. Plasma membrane repair Norma Andrew, Matthias Corrotte and Thiago Castro 20. Magnetic method for lysosome isolation Emyr Lloyd-Evans and Mathew W. Walker
- ISBN: 978-0-12-800079-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 350
- Fecha Publicación: 01/02/2015
- Nº Volúmenes: 1
- Idioma: Inglés