The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon

The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in neuronal cells, and includes sections on such topics as actin transport in axons and neurofilament transport. Covers an increasingly appreciated field in cell biologyIncludes both established and new technologies Contributed by experts in the field INDICE: 1. Axonal Transport: The Orderly Motion of Axonal StructuresMark M Black2. Live-Cell Imaging of Neurofilament Transport in Cultured NeuronsAtsuko Uchida, Paula C. Monsma, J. Daniel Fenn, Anthony Brown3. Axonal Actin in Action: Imaging Actin Dynamics in NeuronsKelsey Ladt, Archan Ganguly, Subhojit Roy4. Live Imaging of Microtubule Dynamics in Organotypic Hippocampal Slice CulturesPhilipp Schätzle, Lukas C. Kapitein, Casper C. Hoogenraad5. Studying Neuronal Microtubule Organization and Microtubule-Associated Proteins Using Single Molecule Localization MicroscopyAnaël Chazeau, Eugene A. Katrukha, Casper C. Hoogenraad, Lukas C. Kapitein6. The Use of Proteomic Analysis to Study Trafficking Defects in AxonsXiaoqin Fu, Kristy J. Brown, Sree Rayavarapu, Kanneboyina Nagaraju, Judy S. Liu7. Using siRNA to Study Microtubule-Related Proteins in Cultured NeuronsLanfranco Leo, Wenqian Yu, Peter W. Baas8. Optimization of Cerebellar Purkinje Neuron Cultures and Development of a Plasmid-Based Method for Purkinje Neuron-Specific, miRNA-Mediated Protein KnockdownC. J. Alexander, J. A. Hammer III9. Biochemical Analysis of Axon-Specific Phosphorylation Events Using Isolated Squid AxoplasmsMinsu Kang, Lisa Baker, Yuyu Song, Scott Brady, Gerardo Morfini10. Analyzing Kinesin Motor Domain Translocation in Cultured Hippocampal NeuronsRui Yang, Marvin Bentley, Chung-Fang Huang, Gary Banker11. Imaging and Quantitative Analysis of Cytokinesis in Developing Brains of Kinesin-6 Mutant MiceKerstin M. Janisch, Noelle D. Dwyer12. Live Cell Imaging of Cytoplasmic Dynein Movement in Transfected Embryonic Rat NeuronsMitchell W. Ross, David J. Mitchell, John C. Cain, Kiev R. Blasier, K. Kevin Pfister13. Live-Cell Imaging of Retrograde Transport Initiation in Primary NeuronsJeffrey J. Nirschl, Erika L. F. Holzbaur14. Methods to Identify and Analyze Gene Products Involved in Neuronal Intracellular Transport Using DrosophilaAmanda L. Neisch, Adam W. Avery, James B. Machamer, Min-gang Li, Thomas S. Hays15. In vivo Analysis of Axonal Transport in ZebrafishCatherine M Drerup, Alex V Nechiporuk16. Fast Axonal Transport in Isolated Axoplasm from the Squid Giant AxonYuyu Song, Minsu Kang, Gerardo Morfini, Scott T. Brady17. Cellular and Subcellular Imaging of Motor Protein-Based Behavior in Embryonic Rat BrainAlexandre D. Baffet, Aurélie Carabalona, Tiago J. Dantas, David D. Doobin, Daniel J. Hu, Richard B. Vallee18. Tracking Quantum-Dot Labeled Neurotropic Factors Transport Along Primary Neuronal Axons in Compartmental Microfluidic ChambersShani Gluska, Michael Chein, Nimrod Rotem, Ariel Ionescu, Eran Perlson19. Studying Endosomes in Cultured Neurons by Live-Cell ImagingZofia M. Lasiecka, Bettina Winckler20. Comparative Analysis of Axonal Transport Markers in Primary Mammalian NeuronsEva Klinman, Erika L. F. Holzbaur21. Imaging Organelle Transport in Primary Hippocampal Neurons Treated with Amyloid-ß OligomersKathlyn J. Gan, Michael A. Silverman22. High Spatial and Temporal Resolution Imaging of Motor-dependent Transport in Neuronal and Nonneuronal CellsJulie Yi, Noopur V. Khobrekar, Tiago J. Dantas, Jie Zhou, Richard B. Vallee23. Isolation and Analyses of Axonal Ribonucleoprotein ComplexesElla Doron-Mandel, Stefanie Alber, Juan A. Oses, Katalin F. Medzihradszky, Alma L. Burlingame, Mike Fainzilber, Jeffery L. Twiss, Seung Joon Le

• ISBN: 978-0-12-803344-9
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 362
• Fecha Publicación: 15/01/2016
• Nº Volúmenes: 1
• Idioma: Inglés