Cellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans, Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino, Methods for detection of mitochondrial reactive oxygen species in senescent cells, Assessment of cell cycle progression and mitotic slippage by videomicroscopy, The original colorimetric method to detect cellular senescence, and more.Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Cell Biology seriesUpdated release includes the latest information on Cellular Senescence and Aging INDICE: 1. Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegansAggeliki Sotiriou, Christina Ploumi, Nikolaos Charmpilas and Nektarios Tavernarakis2. Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholinoSuzita Mohd Noor, Chee Ern David Wong, Pooi-Fong Wong and Anwar Norazit3. Methods for detection of mitochondrial reactive oxygen species in senescent cellsFenniche Salma, Oubaddou Yassire, Bakri Youssef, Dupuy Corinne and Ameziane El Hassani Rabii4. Assessment of cell cycle progression and mitotic slippage by videomicroscopyLuca Mattiello, Sara Soliman Abdel Rehim, Gwenola Manic and Ilio Vitale5. The original colorimetric method to detect cellular senescenceManjari Dimri and Goberdhan P. Dimri6. Assessing microbiota composition in the context of agingDiana Campos-Iglesias, José M.P. Freije and Carlos López-Otín7. Assessing chronological aging in Saccharomyces cerevisiaeAdina Schulze, Andreas Zimmermann, Katharina Kainz, Nadine B. Egger, Maria A. Bauer, Frank Madeo and Didac Carmona-Gutierrez8. Image processing and supervised machine learning for retinal microglia characterization in senescenceSoyoung Choi, Daniel Hill, Jonathan Young and Maria Francesca Cordeiro9. Measuring telomerase activity using TRAP assaysGabriele Saretzki10. High throughput assessment of cellular senescenceGiulia Cerrato, Allan Sauvat, Felix Peyre, Oliver Kepp and Guido Kroemer11. Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive eventsV. Paget, O. Guipaud, A. François and F. Milliat12. Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP)Nicolas Malaquin and Francis Rodier13. Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitorsVanessa Klapp, Norma Bloy, Carlos Jiménez-Cortegana, Aitziber Buqué and Giulia Petroni14. Mechanistic exploration of autophagy and aging by RNA interferenceHui Pan, Oliver Kepp and Guido Kroemer
- ISBN: 978-0-443-18896-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 250
- Fecha Publicación: 12/01/2024
- Nº Volúmenes: 1
- Idioma: