Dielectrophoresis: Theory, Methodology and Biological Applications

Comprehensive coverage of the  basic theoretical concepts and applications of dielectrophoresis from a world–renowned expert. Features hot application topics including: Diagnostics, Cell–based Drug Discovery, Sensors for Biomedical Applications, Characterisation and Sorting of Stem Cells, Separation of Cancer Cells from Blood and Environmental Monitoring Focuses on those aspects of the theory and practice of dielectrophoresis concerned with characterizing and manipulating cells and other bioparticles such as bacteria, viruses, proteins and nucleic acids. Features the relevant chemical and biological concepts for those working in physics and engineering INDICE: Preface .Nomenclature .1. Placing Dielectrophoresis into Context as a Particle Manipulator .1.1 Introduction .1.2 Characteristics of Micro–Scale Physics .1.3 Microfluidic Manipulation and Separation of Particles .1.4 Candidate Forces for Microfluidic Applications .References .2. How does Dielectrophoresis differ from Electrophoresis? .2.1 Introduction .2.2 Electric Field .2.3 Electrophoresis .2.4 Induced Surface Charge and Dipole Moment .2.5 Dielectrophoresis .2.6 Summary .References .3. How does Dielectrophoresis differ from Electrophoresis? .3.1 Introduction .3.2 Charges and Fields .3.3 Gauss s Law .3.4 Induced Dielectric Polarisation .3.5 Capacitance .3.6 Divergence Theorem and Charge Density Relaxation Time .3.7 Summary .References .4. Electrical Potential Energy and Electric Potential .4.1 Introduction .4.2 Electrical Potential Energy .4.3 Electrical Potential .4.4 Electrostatic Field Energy .4.5 Summary .References .5. Potential Gradient, Field and Field Gradient; Image Charges and Boundaries .5.1 Introduction .5.2 Potential Gradient and Electrical Field .5.3 Applying Laplace s Equation .5.4 Method of Image Charges .5.5 Electric Field Gradient .5.6 Electrical Conditions at Dielectric Boundaries .5.7 Summary .References .6. The Clausius–Mossotti Factor .6.1 Introduction .6.2 Development of the Clausius–Mossotti–LorentzRelation .6.3 Refinements of the Clausius–Mossotti–LorentzRelation .6.4 The Complex Clausius–Mossotti Factor .6.5 Summary .6.6 References .7. Dielectric Polarisation .7.1 Introduction .7.2 Electrical Polarisation at the Atomic and Molecular Levels .7.4 Dipole Relaxation and Energy Loss .7.5 Interfacial Polarisation .7.6 Summary .References .8. Dielectric Properties of Water, Electrolytes, Sugars, Amino–Acids, Proteins and Nucleic Acids .8.1 Introduction .8.2 Water .8.3 Electrolyte Solutions .8.4 Amino Acids and Proteins in Solution .8.5 Nucleic Acids .8.6 Summary .References .9. Dielectric Properties of Cells .9.1 Introduction .9.2 Cells: A Basic Description .9.3 Electrical Properties of Cells .9.4 Modelling the Dielectric Properties of Cells .9.5 Effect of Cell Surface Charge on Maxwell–Wagner Relaxation .9.6 Dielectric Properties of Bacteria .9.7 Summary .References .10. Dielectrophoresis: Theoretical and Practical Considerations .10.1 Introduction .10.2 Inherent Approximations in the DEP Force Equation .10.3 Refinements of the DEP Force Equation .10.4 Electrodes: Fabrication, Materials and Modeling .10.5 The Second (High–Frequency) DEP Cross–Over Frequency (fxo2) .10.6 Summary .References .11. Dielectrophoretic Studies of Bioparticles .11.1 Introduction .11.2 DEP Characterisation and Separation of Live and Dead Cells .11.3 Mammalian Cells .11.4 Bacteria .11.5 Other Cell Types (Plant, Algae, Oocytes, Oocysts) and Worms .11.6 Virions .11.7 Nucleic Acids and Proteins .11.8 Summary .References .12. Microfluidic Concepts of Relevance to Dielectrophoresis .12.1 Introduction .12.2 Gases and Liquids .12.3 Fluids treated as a Continuum .12.4 Basic Fluid Statics and Fluid Dynamics .12.5 Navier–Stokes Equations .12.6 Diffusion .12.7 Ionic (Electrical) Double Layer .12.8 Electroosmosis .12.9 Summary .12.10 References .Appendices .A: Values of Fundamental Physical Constants .B: SI Prefixes .C: The base quantities in the SI system of units .D: Derived Physical Quantities, their Defining Equation or Law, and Dimensions .E: Diffusion Coefficients for Molecules and Ions in Water at 298 K .F: Diffusion Coefficients for Bio–Particles in Water at 293 K .G: Viscosity and Surface Tension Values for Liquids at 293 K .H: Activity Coefficients for Common Compounds that Dissociate into Ions in Solution .I: Electrical Mobility of Ions at 25oC in Dilute Aqueous Solution .J: Buffering Systems and their pH Buffering Range .K: Composition of 1–L of Human Blood .L: Blood Cells, Platelets and some Pathogenic Bioparticles

• ISBN: 978-1-118-67145-0
• Editorial: Wiley–Blackwell
• Encuadernacion: Cartoné
• Páginas: 448
• Fecha Publicación: 28/04/2017
• Nº Volúmenes: 1
• Idioma: Inglés