De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. Continues the legacy of this premier serial with quality chapters on de novo enzyme designRepresents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism INDICE: 1. Computational Protein Design David Baker and Daniel Adriano Silva Manzano 2. Chemical post-translational modification with designed rhodium(II) catalysts Zachary Ball3. Ligand Binding Assays for Determining the Affinity of Protein-Protein Interactions: Technologies and Considerations Jennifer R. Cochran4. Incorporation of non-natural amino acids into proteins Irene Coin5. Electron Transfer in Protein Maquettes Les Dutton6. Designing Peptide Based Redox Catalysts Giovanna Ghirlanda7. Designing Metal Binding Maquettes Brian R. Gibney8. Insertion of non-natural cofactors into proteins Takashi Hayashi9. Using SpyTag/SpyCatcher covalent ligation to cyclize enzymes Mark Howarth10. Solid Phase Synthesis of Aromatic Oligoamide Foldamers Ivan Huc11. Designing Cu metallopeptides Olga Iranzo12. Non-covalent Interactions in peptide assemblies Alan Kennan13. Functional B-sheet Peptides Ivan Korendovych14. Peptide Binding for Bio-based Nanomaterials Marc Knecht15. Cysteine bis-alkylation as a means of conformational restriction of peptide epitopes Joshua Kritzer16. Designing Photoactivatable proteins Brian Kuhlman17. Combinatorial Approaches for Protein Design Jonathan Robert Lai18. Designed Four Helix Bundles: From Structure to Functions Angela Lombardi19. Designing Heteronuclear Metalloenzymes Yi Lu20. Designing Fluorinated Peptides E Neil G. Marsh21. De Novo Designed Imaging Agents for the Clinic Anna Peacock22. Structural Solution of Highly Symmetric Three Stranded Coiled Coils Vincent Pecoraro23. Protein-Protein Interaction Design Lynne Regan24. The design of peptide bundles that fold and Alanna Schepartz25. Chemically Tunable Assembly of Protein Superstructures F. Akif Tezcan26. Inserting Non-natural Amino Acids into Proteins Jiangyun Wang27. Artificial metalloenzyme compartmentalization in the periplasm of E. coli Thomas R. Ward28. Parametric design of alpha-helical bundles and barrels Drew Thomson and Dek Woolfson 29. TBC Hiroshi Nakajima
- ISBN: 978-0-12-805380-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/09/2016
- Nº Volúmenes: 1
- Idioma: Inglés