Vibrational Spectroscopy in Protein Research

In recent years, a range of new, state-of-the art vibrational spectroscopic techniques have been developed, including Raman imaging, Raman Optical Activity (ROA), SERS, TERS, non-linear Raman spectroscopy, AFM-IR, Cascade laser IR spectroscopy, IR imaging, NIR imaging, Terahertz spectroscopy, and time-resolved vibrational spectroscopy. In addition, spectral analysis methods, such as chemometrics and quantum chemical calculation, are now seeing wider use by researchers across the biomedical and life sciences.  Vibrational Spectroscopy in Protein Research: From Purified Proteins to Aggregates and Assemblies offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for analysis of purified proteins, chapter authors also examine use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins, and protein assemblies. Emphasis throughout is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies. Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the fieldEmphasizes in vivo, in situ, and non-invasive analysis of proteins in biomedical and life science research more broadlyChapters address vibrational spectroscopy not only for the study of simple purified proteins but protein aggregates, fibrous proteins, membrane proteins, and protein assemblies INDICE: 1. Vibrational imaging of proteins; changes in the tissues and cells in life style diseases studies 2. Detection of proteins associated with disease using vibrational spectroscopy 3. NIR Imaging studies of proteins in fish egg development 4. Raman studies of fibrous proteins 5. AFM-IR on protein aggregation 6. Two-dimensional correlation spectroscopy of proteins 7. FT-IR studies of photosynthesis proteins 8. Secondary structure of proteins studied by ROA 9. Single proteins placed within the SERS hot spots of self-assembled silver nanolenses 10. ATR-IR imaging of proteins 11. TERS of DNA and proteins 12. DUV resonance studies of proteins 13. VCD of protein fibrils 14. Biomedical application of vibrational spectroscopy: proteins 15. Probing proteins with UV resonance Raman spectroscopy 16. ROA of proteins 17. Nonlinear correlation spectroscopy of proteins 18. Multidimensional Infrared Spectroscopy for elucidating protein structure and function (dynamics) 19. Model Protein Embedded in Solid Lipid 20. SERS In vivo intracellular monitoring and imaging of relevant cytoplasm metabolites under different conditions 21. Molecular spectroscopy studies of molecular changes that occur during disease progression and to instigate rapid objective biofluid diagnostics

• ISBN: 978-0-12-818610-7
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 480
• Fecha Publicación: 01/01/2020
• Nº Volúmenes: 1
• Idioma: Inglés