This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceProvides invaluable details on state-of-the-art methods to assess a broad array of mitochondrial functions INDICE: 1. Measurement of ROS homeostasis in isolated mitochondria2. Use of Potentiometric Fluorophores in the measurement of mitochondrial ROS production3. Methods of measuring ROS including spatial, temporal and quantitative manipulation of ROS production4. Imaging changes in the cytosolic ATP-to-ADP ratio5. The design, implementation, and interpretation of Seahorse extracellular flux experiments in neural cells. 6. The use of miniSOGs in the localization of mitochondrial proteins7. The use of mitoTALENS as potential therapy for mitochondrial disease8. The use of 18F-BCPP-EF as a PET probe for Complex I activity in the brain9. Measurement of cerebral oxygen extraction fraction using MRI in patients with MELAS 10. Measurement of the function of mitochondrial-associated membranes11. Measurement of mitochondrial trafficking and life cycle using MitoTimer12. Analysis of mitochondrial traffic in Drosophila13. Characterization of mitochondrial transport in neurons14. In vivo tracking of mitochondria in zebrafish15. Mitochondrial trafficking in mouse models of motor and sensory neuropathies16. Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures17. High-throughput screening for Parkin regulators18. Measurement of mitophagy19. Measurement of mitochondrial fusion by photoactivated fluorophores20. Assessment of mitochondria in pluripotent stem cell differentiation21. Iron in mitochondria
- ISBN: 978-0-12-801415-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 07/11/2014
- Nº Volúmenes: 1
- Idioma: Inglés