Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Cell Biology seriesIncludes the latest information on the topic of development, characterization and applications in CAR T Cells INDICE: 1. How to Apply the Broad Toolbox of Correlative Light and Electron Microscopy to Address a Specific Biological QuestionThomas Müller-Reichert, Erin M. Tranfield, Gunar Fabig and Thomas Kurth2. Some tips and tricks for a correlative light electron microscopy workflow using stable expression of fluorescent proteinsPaul Verkade3. Targeting membrane receptors with fluoronanogold probes for high resolution correlative microscopy.Monica Fernandez Monreal4. Correlative light and electron microscopy at defined cell cycle stages in a controlled environmentShotaro Otsuka5. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopyPaul Verkade, Maximillian Erdmann, Isobel Web, Lorna Hodgson and Andrew Davidson6. Tissue CLEMChristine Longin7. Array tomography of in vivo labeled synaptic receptorsChristian Stigloher and Sebastian Britz8. Correlative cryo-microscopy pipelines for in situ cellular studiesAnna Sartori and Chiara Zurzolo9. Building a super-resolution fluorescence cryomicroscopeThom Sharp10. Analysis of super resolution CLEM dataThom Sharp11. Nanometer targeting using cryo super resolutionLaura Carolina Zanetti-Domingues12. Laboratory Based Correlative Cryo-Soft X-Ray Tomography and Cryo-Fluorescence MicroscopyKenneth Fahy
- ISBN: 978-0-323-95141-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 336
- Fecha Publicación: 30/04/2024
- Nº Volúmenes: 1
- Idioma: Inglés