All set to become be the ultimate reference on this growing technique, this text provides a didactic overview of the fundamentals while showing in detail how it is applied in a wide range of hot interdisciplinary applications. With its unified, well–structured approach, this is the perfect guide for both newcomers and advanced practitioners. INDICE: BACKGROUND, THEORY AND CONCEPTS INTRODUCTION Origins and History Theodor Förster (Life and Scientific Contribution) FÖRSTER THEORY Classical Theory Quantum Mechanical Theory Other Energy Transfer Mechanisms OVERVIEW OF HOW TO APPLY FRET The basic FRET concept Förster Distance / Förster Radius Efficiency and Transfer Rates Examples of Experimental FRET Calculations Steady–State FRET Measurements Time–Resolved FRET Measurements Frequency–domain FRET Measurements FRET beyond Förster?s Theory THE FRET TOOLBOX – MATERIALS Donors / Acceptors (Advantages and Disadvantages) COMMON FRET TECHNIQUES/APPLICATIONS SENSING AND DIAGNOSTICS (IN VITRO) Sensors with unlinked donor–acceptor pairs (FRET due to molecular collision) Sensors with linked donor–acceptor pairs (FRET due to molecular recognition) Solid phase sensing Liquid phase sensing Physiological sensing (pH, ions, oxygen, CO2 etc.) Analytical sensing (diagnostic assays) SINGLE MOLECULE APPLICATIONS Direct single molecule studies spFRET FRET IN THE DETERMINATION OF PROTEIN, PEPTIDE AND OTHER BIOLOGICAL STRUCTURES (PRINCIPALLY SPECTROSCOPIC RULER APPLICATIONS) Structural investigation Conformational Investigation Dynamic Investigations (e.g. protein folding) IN VIVO IMAGING APPLICATIONS Cellular studies Small Animal and other studies HIGH–THROUGHPUT AND POINT–OF–CARE APPLICATIONS The importance of fast and small applications Time–Resolved Techniques Multiplexed Techniques FRET WITH RECENTLY DEVELOPED MATERIALS FLUORESCENT PROTEINS Brief description of origins Available proteins and engineering of biomolecules with fluorescent protein labels First usage in FRET Representative applications SEMICONDUCTOR QUANTUM DOTS Brief description of origins Labeling QDs for FRET First usage in FRET Representative applications FRET IN NANOTECHNOLOGY Dendrimers Light Collection (Antenna Systems) Photonic wires SUPPORTING INFORMATION AND CONCLUSIONS IMPORTANT FRET DATA Donor–Acceptor Pair Data (Förster Distances, Overlap Integrals, Quantum Yields etc.) Experimental Equipment HELPFUL FRET INFORMATION FRET in the Literature FRET on the Web FRET Research Groups Commercial FRET applications / suppliers OUTLOOK ON FRET (THE FUTURE OF RESONANCE ENERGY TRANSFER)
- ISBN: 978-3-527-32816-1
- Editorial: Wiley VCH
- Encuadernacion: Cartoné
- Páginas: 816
- Fecha Publicación: 13/11/2013
- Nº Volúmenes: 1
- Idioma: Inglés