T-cell trafficking: methods and protocols

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explorehow the development of novel and cutting-edge techniques, particularly in thefield of real-time imaging and genetic manipulation, have led to an increasedunderstanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions invitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responsesin experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which sharestips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories. Includes detailed description of cutting edge imaging techniques, including multiphoton microscopy, and software-based analysis of T cell infiltration in vivo Describes methods for setting up assays for the observation of lymphocyte migration under flow in vitro Provides description of effective genetic manipulation of the endothelium Includes assays for the investigation of recently described migratory mechanisms, including fugetaxis INDICE: How T cells find their way around.- Live imaging of leukocyte-endothelium interactions.- Leukocyte adhesion under haemodynamic flow conditions.-Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells.- Discriminating between the paracellular and transcellular routes of diapedesis.- Monitoring RhoGTPase activity in lymphocytes.- Visualisation of Signalling in Immune Cells.- Methods for quantisation of lymphocyte chemotaxis and fugetaxis.- Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes.- Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells.- Visualisation of lymphoid organ development.- Single Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy.- Imaging interactions between the immune and cardiovascular systems in vivoby multiphoton microscopy.- Applying an adaptive watershed to the tissue cellquantification during T-cell migration and embryonic development.- Identifying homing interactions in T cell traffic in human disease.- Tracking antigen experienced effector T cells in vitro and in vivo.- Pre-clinical testing of strategies for therapeutic targeting of human T cell trafficking in vivo.

• ISBN: 978-1-60761-460-9
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 246
• Fecha Publicación: 01/02/2010
• Nº Volúmenes: 1
• Idioma: Inglés