Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagentsExplains basic laboratory preparationProvides straightforward experimental protocolsIncorporates fundamental analytical techniquesContains a troubleshooting guide INDICE: IntroductionChapter One: Things to consider before planning your first PCR Chapter Two: Designing your primersChapter Three: Extracting DNA/RNAChapter Four: Choosing the basic parametersChapter Five: Analysing your first PCRChapter Six: Troubleshooting your first PCRChapter Nine: RT-PCR - things to considerChapter Ten: Carrying out your first RT-PCRChapter Eleven: Analysing your first RT-PCRChapter Twelve:Ttroubleshooting your RT-PCRAppendices - to include recommended suppliers and websites
- ISBN: 978-0-12-802683-0
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 224
- Fecha Publicación: 01/08/2016
- Nº Volúmenes: 1
- Idioma: Inglés