Analyzing Biomolecular Interactions by Mass Spectrometry

This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS–based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down–based approaches, this section also emphasizes the use of ion mobility MS, capture–compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays.For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories. INDICE: Introduction to Mass Spectrometry.DIRECT MS BASED AFFINITY TECHNIQUES.Studying Protein–Protein Interactions by Combining Native Mass Spectrometry and Chemical Cross–Linking.Native Mass Spectrometry Approaches Using Ion–Mobility MS.LC–MS BASED WITH INDIRECT ASSAYS.Methodologies for Effect–Directed Analysis: Environmental Applications, Food Analysis and Drug Discovery .MS Binding Assays.Metabolic Profiling Approches for the Identification of Bioactive Metabolites in Plants.Antivenomics: A Proteomics Tool for Studying the Immunoreactivity of Antivenoms.DIRECT PRE– AND ON–COLUMN COUPLED TECHNIQUES.Frontal and Zonal Affinity Chromatography Coupled to Mass Spectrometry.On–Line Affinity Assessment and Immunoaffinity Sample Pretreatment in Capillary Electrophoresis–Mass Spectrometry.Label–Free Biosensor Affinity Analysis Coupled to Mass Spectrometry.DIRECT POST COLUMN COUPLED AFFINITY TECHNIQUES.High–resolution Screening: Post–Column Continuous–Flow Bioassays.Conclusions and Perspectives.

• ISBN: 978-3-527-33464-3
• Editorial: Wiley VCH
• Encuadernacion: Cartoné
• Páginas: 400
• Fecha Publicación: 25/03/2015
• Nº Volúmenes: 1
• Idioma: Inglés