The objective of this volume of Methods in Molecular Biology on Structural Proteomics: High-throughput methods is to provide readers with a current view ofall aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. The readers are encouraged to access further details on the methodologies in on-line resources such as PepcDB (Protein expression purification and crystallization database; pepcdb.pdb.org) and in the cited literature. We emphasize that most methods are as amenable to small laboratories as to large consortia, and do not require major investments in facilities; we hope that this volume will help smaller laboratories establish some of the high-throughput techniques. First book ofprotocols to cover techniques in a new and emerging field INDICE: From the contents Target selection for structural genomics: An Overview.- A General target selection method for crystallographic proteomics.- Target Selection: Triage in the Structural Genomics Battlefield.- Data Management in Structural Genomics: An Overview.- Data deposition and annotation at the Worldwide Protein Data Bank.- Prediction of protein disorder.- Protein domain prediction.- Protein Structure Modeling With MODELLER.- High throughput cloning with restriction enzymes.- Automated recombinant protein expression screening in Escherichia coli.- From no expression to high-level soluble expression inEscherichia coli by screening a library of the target protein with randomizedN-terminus.- Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli.- A high-throughput platform for eukaryotic genes.- High-Throughput production of recombinant human proteins for crystallography.- Assembly of protein complexes by co-expression in prokaryotic and eukaryotic hosts.
- ISBN: 978-1-58829-809-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 400
- Fecha Publicación: 01/02/2008
- Nº Volúmenes: 1
- Idioma: Inglés