Isotope Labeling of Biomolecules - Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on heavy-isotope labeling methods and applications for biomolecules. Contains contributions from leading authorities in the field of isotope labeling of biomolecules Informs and updates on the latest developments in the fieldProvides comprehensive information on heavy-isotope labeling methods and applications for biomolecules INDICE: 1. Selective amino acid segmental labeling of multidomain proteins2. RNA segmental labeling for NMR and EPR3. Evaluation of higher order structure of protein therapeutics using two dimensional NMR at 13C and 15N natural isotopic abundance4. Amino acid selective unlabeling for NMR-based structural studies of proteins5. Using istopically labeled mAb's in PK/PD studies6. Labeling of membrane proteins by cell-free expression7. Application of the thymine isostere 2,4-Difluoro-5-methylbenzene as a tool for NMR assignment and analysis of nucleic acids8. Isotope labeling of eukaryotic membrane proteins in yeast9. Essential deuteration strategies for neutron protein dynamics experiments (spin echo)10. Labeled protein expression in Halobacterium salinarum11. Intein based segmental labeling of proteins12. Chemo-enzymatic synthesis of site-specifically and isotopically labeled nucleotides and phosphoramidites to faciliate NMR dynamics and structure13. Large scale expression and purification of site-specific isotopically labeled recombinant RNA in Escherichia coli variants for NMR studies14. Deuteration for neutron scattering I: Crystallography15. A rapid and robust method for selective isotope labeling of proteins16. Protein labeling in insect cells17. Fluorinated proteins18. Deuteration in Biological Neutron Reflectometry19. 19F-site specific labeled nucleotides for nucleic acid structural analysis by NMR20. Robust high yield methodologies for 2H and 2H/15N/13C labelling of proteins for structural investigations using neutron scattering and NMR21. Metabolic control of deuterium labelling of biopolyesters and polysaccharides for structure and dynamics investigations using Small angle Neutron Scattering, NMR and IR microspectroscopy22. Deuterium labelling strategies for creating contrast in structure-function studies of lipid bilayers, and assembled biosensors, using neutron reflectometry23. Expression and purification of single, double and triple labeling of proteins in bacteria and yeast24. The characterization of protein-protein interactions by HDX-MS25. Deuteration of proteins for contrast variation application in SANS26. The use of HDX-MS for studies of viruses and viral proteins27. Expression of isotope labeled peptides for targeted proteomics using reconstituted protein translation system28. HDX-MS as a tool to study protein dynamics29. Development of approaches for deuterium incorporation in plants30. Deuterated cellulosics for structural studies31. Use of isotope-labeled internal standard as reference material for clinical diagnostics32. Methyl-groups labeling of amino acids33. In vitro transcription/translation as a tool for protein labeling34. Labeling of proteins in mammalian cells35. Labeling and purification of DNA repair Enzymes36. Isotopic Labeling of Carbohydrates
- ISBN: 978-0-12-803048-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 01/11/2015
- Nº Volúmenes: 1
- Idioma: Inglés