Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. This practical guide to biomolecular labeling looks at new techniques that are becoming widely used. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldFocuses on stable isotope labeling of biomolecules, which is important for structural studies of proteins and nucleic acids INDICE: 1. Selective amino acid segmental labeling of multidomain proteins2. RNA segmental labeling for NMR and EPR3. Evaluation of higher order structure of protein therapeutics using two dimensional NMR at 13C and 15N natural isotopic abundance4. Amino acid selective unlabeling for NMR-based structural studies of proteins5. Using istopically labeled mAb's in PK/PD studies6. Labeling of membrane proteins by cell-free expression7. Application of the thymine isostere 2,4-Difluoro-5-methylbenzene as a tool for NMR assignment and analysis of nucleic acids8. Isotope labeling of eukaryotic membrane proteins in yeast9. Essential deuteration strategies for neutron protein dynamics experiments (spin echo)10. Labeled protein expression in Halobacterium salinarum11. Intein based segmental labeling of proteins12. Chemo-enzymatic synthesis of site-specifically and isotopically labeled nucleotides and phosphoramidites to faciliate NMR dynamics and structure13. Large scale expression and purification of site-specific isotopically labeled recombinant RNA in Escherichia coli variants for NMR studies14. Deuteration for neutron scattering I: Crystallography15. Deuteration for neutron scattering II: Solutions & partially ordered systems16. Deuteration for neutron scattering III: Dynamics17. Lipid labeling18. A rapid and robust method for selective isotope labeling of proteins19. Protein labeling in insect cells20. Fluorinated proteins21. Deuteration in Biological Neutron Reflectometry22. 19F-site specific labeled nucleotides for nucleic acid structural analysis by NMR23. Robust high yield methodologies for 2H and 2H/15N/13C labelling of proteins for structural investigations using neutron scattering and NMR24. Metabolic control of deuterium labelling of biopolyesters and polysaccharides for structure and dynamics investigations using Small angle Neutron Scattering, NMR and IR microspectroscopy25. Deuterium labelling strategies for creating contrast in structure-function studies of lipid bilayers, and assembled biosensors, using neutron reflectometry26. Expression and purification of single, double and triple labeling of proteins in bacteria and yeast27. The characterization of protein-protein interactions by HDX-MS28. Deuteration of proteins for contrast variation application in SANS29. The use of HDX-MS for studies of viruses and viral proteins30. Expression of isotope labeled peptides for targeted proteomics using reconstituted protein translation system31. Stable isotope labeling strategies for NMR investigations of proteins32. HDX-MS as a tool to study protein dynamics33. Development of approaches for deuterium incorporation in plants34. Deuterated cellulosics for structural studies35. Fed-batch fermentation for growing E. coli in D2O36. Use of isotope-labeled internal standard as reference material for clinical diagnostics37. Methyl-groups labeling of amino acids38. In vitro transcription/translation as a tool for protein labeling39. Labeling of proteins in mammalian cells40. Labeling and purification of DNA repair Enzymes41. Isotopic Labeling of Carbohydrates
- ISBN: 978-0-12-803049-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 08/01/2016
- Nº Volúmenes: 1
- Idioma: Inglés