Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Describes abreadth of uniquely suited model systems for analysis of chemotaxis, including bacteria, Dictyostelium, yeast, Drosophila, zebrafish, neurons, and immune and tumor cells Examines phenomena related to chemotaxis, including chemotropism, phototaxis, and electrotaxis Details the application of cutting-edge technologies for stimulating cells with chemoattractants and analyzing their resulting cellular behaviors INDICE: From the contents Microfluidic Techniques for the Analysis of Bacterial Chemotaxis.- Prokaryotic Phototaxis.- Photoorientation in PhotosyntheticFlagellates.- Dictyostelium Slug Phototaxis.- Electrotaxis and Wound Healing:Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration.- Chemotropism During Yeast Mating.- Group Migration and Signal Relay In Dictyostelium.- Quantitative Analysis of Distal Tip Cell Migration in C. elegans.- Inflammation and Wound Healing in Drosophila.- Neutrophil MotilityIn Vivo Using Zebrafish.- Chemotaxis in Neutrophil-like HL-60 Cells.- Chemokine Receptor Dimerization and Chemotaxis.- Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes.- Breast Cancer CellMovement: Imaging Invadopodia by TIRF and IRM Microscopy.- In vivo Assay for Tumor Cell Invasion.- Quantitative Studies of Neuronal Chemotaxis in 3D.
- ISBN: 978-1-60761-197-4
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 510
- Fecha Publicación: 01/09/2009
- Nº Volúmenes: 1
- Idioma: Inglés