Basic Science Methods for Clinical Researchers

Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background, who have a lack of exposure to basic science due to heavy clinical demands weighing on their time. The aim is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science backgroundOrganized around research themes pertaining to key biological molecules, from genes to proteins to cells, and model organismsFeatures protocols, techniques for trouble-shooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive dataAppendices provide resources for practical research methodology including legal frameworks for using stem cells and animals in the laboratory, ethical considerations and good laboratory practice (GLP) INDICE: PrefaceSection I: Gene Chapter 1: PCR, qPCR, and RT-PCRChapter 2: Methods of CloningChapter 3: In situ HybridisationChapter 4: MicroarraySection II: ProteinChapter 5: Western BlotChapter 6: Enzyme-Linked Immunosorbent AssayChapter 7: ImmunofluorescenceSection III: Cell Chapter 8: Cell CultureChapter 9: Flow CytometryChapter 10: TransfectionChapter 11: Functional AssaysSection IV: System Chapter 12: In vivo Animal Modelling: DrosophilaChapter 13: In vivo Animal Modelling: DanioChapter 14: In vivo Animal Modelling: XenopusChapter 15: In vivo Animal Modelling: MouseAppendicesAppendix A: Legal framework on the scientific use of animals in researchAppendix B: Regulatory framework of stem cell research

• ISBN: 978-0-12-803077-6
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 256
• Fecha Publicación: 01/05/2016
• Nº Volúmenes: 1
• Idioma: Inglés