Proteomic and Metabolomic Approaches to Biomarker Discovery

Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery, Second Edition covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. This second edition has been fully updated and revised to address recent advances in MS and NMR instrumentation, high-field NMR, proteomics and metabolomics for biomarker validation, clinical assays of biomarkers and clinical MS and NMR, identifying microRNAs and autoantibodies as biomarkers, MRM-MS assay development, top-down MS, glycosylation-based serum biomarkers, cell surface proteins in biomarker discovery, lipodomics for cancer biomarker discovery, and strategies to design studies to identify predictive biomarkers in cancer research. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. Fully updated to address the full range of proteomic and metabolomic methods and technologies used for biomarker discovery and validationCovers all steps involved in biomarker discovery, from study design to study executionServes as a vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students INDICE: 1. Biomarker Discovery: Study Design and Execution2. Proteomic Technologies for Biomarker Discovery3. Sample Preparation for Proteomics4. Sample preparation For metabolomics 5. SOP for serum and plasma 6. Current NMR Strategies7. Data-independent Mass Spectrometry Extends Detectable Dynamic Range 8. GC/MS 9. LC/MS for: (a) Metabolomics 10. CE/MS for (a) Proteomics (b) Metabolomics 11. Gel electrophoresis 12. The Role of Two-dimensional Gel Electrophoresis in Biomarker Discovery13. Affinity 14. Asp selective Microwave-supported acid proteolysis 15. Fractionation of complex mixtures16. Protein and Metabolite Identification17. Quantitative proteomics 18. Quantitative analysis of metabolites in biological fluids 19. Statistical & computational analysis 20. Top-down Proteomics for Biomarker Discovery21. A role for protein-protein interaction networks in the identification and characterization of potential biomarkers 22. Protein microarrays 23. Autoantibodies as biomarkers24. MicroRNAs and Biomarker Discovery25. Tissue imaging for biomarker discovery26. MS approaches for biomarker verification 27. MS metabolomic data handling for biomarker discovery28. Biomarker Validation

• ISBN: 978-0-12-818607-7
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 524
• Fecha Publicación: 01/01/2020
• Nº Volúmenes: 1
• Idioma: Inglés