Epigenetic Biomarkers and Diagnostics comprises 32 chapters contributed by leading active researchers in clinical epigenetics. The book is divided into three sections, beginning with the basis of epigenetic mechanisms and descriptions of epigenetic biomarkers that can be used in clinical diagnostics and prognostics. The second section refers to the importance of the sample quality and ethical issues in clinical epigenetics, with the final section presenting the technologies and methods to discover and analyze epigenetic biomarkers. The book describes the challenging aspects of epigenetic research and current findings regarding new epigenetic elements and modifiers, providing guidance for researchers interested in the most advanced technologies and methodologies used in the clinical diagnosis or prognosis of disease. Focuses on recent progress in several areas of epigenetics, general concepts regarding epigenetics, and the future prospects of this discipline in clinical diagnostics and prognosticsDescribes the importance of the quality of samples and clinical associated data, and also the ethical issues for epigenetic diagnosticsDiscusses the advances in epigenomics technologies, including next-generation sequencing based tools and applicationsExpounds on the utility of epigenetic biomarkers for diagnosis and prognosis of several diseases, highlighting the study of these biomarkers in cancer, cardiovascular and metabolic diseases, infertility, and infectious diseasesIncludes a special section that discusses the relevance of biobanks in the maintenance of high quality biosamples and clinical-associated data, and the relevance of the ethical aspects in epigenetic studies INDICE: Chapter 1. Overview: Epigenetics. New findings, perspectives and future directions SECTION 1. EPIGENETIC BIOMARKERS AND CLINICAL APPLICATIONS DNA methylation and hydroxymethylation Chapter 2. DNA methylation biomarkers in lung cancer Chapter 3. DNA methylation in prostate cancer Chapter 4. DNA methylation in breast cancer Chapter 5. DNA methylation and metabolic syndrome, diabetes and obesity Chapter 6. DNA methylation biomarkers in asthma and allergy Chapter 7. DNA methylation analysis in preeclampsia and other pregnancy complications Post-translational modifications and histone variants Chapter 8. Overview: Histone code and disease, the use of post-translational modifications in clinical diagnosis Chapter 9. Histone post-translational modifications in prostate cancer Chapter 10. Histone post-translational modifications in breast cancer and their use in clinical diagnosis and prognosis miRNAs Chapter 11. Overview: The use of non-coding RNAs in diagnosis: miRNAs, lncRNAs, and circularRNAs. Chapter 12. miRNAs in prostate cancer and their value as biomarkers Chapter 13. miRNAs in breast cancer and their value as biomarkers Chapter 14. Biomarkers and diagnostics based on miRNAs in human sarcoma Chapter 15. miRNAs in the physiopathology of diabetes and their value as biomarkers Chapter 16. Long Non-coding RNAs and their role in complex human diseases SECTION 2: EPIGENETIC BIOMARKERS: QUALITY OF SAMPLES AND ETHICAL ISSUES Chapter 17. The importance of Biobanks and registries for high throughput epigenetic studies Chapter 18. Ethical aspects of epigenetic studies and epidemiological epigenetics SECTION 3: TECHNOLOGIES AND METHODS IN EPIGENETICS: FROM BASIC AND CLINICAL RESEARCH TO DIAGNOSTICS Chapter 19. Overview: The revolution of the new technologies and methods in the epigenetic research of DNA methylation Chapter 20. DNA methylation studies using Next Generation Sequencing Chapter 21. DNA methylation studies using Sequenom Histone Post-translational modifications Chapter 22. Mass spectrometry for the identification of post-translational modifications in histones and its application in clinical epigenetics microRNAs Chapter 23. miRNAs and lncRNAs analysis by NGS platforms and its application in clinical epigenetics Chapter 24. A novel approach to early diagnostics: Universal Screening Test (UST) based on analysis of organ-enriched circulating microRNAs
- ISBN: 978-0-12-801899-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 464
- Fecha Publicación: 01/11/2015
- Nº Volúmenes: 1
- Idioma: Inglés