Post-translational Modifications that Modulate Enzyme Activity

Post-translational Modifications that Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on Post-translational Modifications that Modulate Enzyme Activity INDICE: 1. Crosstalk between cellular metabolism and histone acetylation Nathaniel Snyder 2. Isolation of protein complexes and modifications that regulate transcriptional machinery Michael Washburn 3. High-throughput phosphoproteome mapping through multiplexed mass spectrometry Wilhelm Haas 4. Differentiation of D and L epimerization in proteins Ryan Julian 5. Biochemical analysis of protein arginylation Anna Kashina 6. Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale Yue Chen 7. Genomic and biochemical analysis of RNA post-transcriptional modifications Fange Liu 8. Isolation and characterization of glycosylated (neuro)peptides Lingjun Li 9. Global detection of protein sialylation and phosphorylation by mass spectrometry Martin R. Larsen 10. Split Protein trans-splicing for creation of site-specifically modified proteins in vivo Yael David 11. Global and site-specific analysis of protein N-glycosylation with mass spectrometry (MS)-based proteomics methods Ronghu Wu 12. Small molecule interrogation of the glycome Mia Huang 13. Probing the structure and function of N-terminal acetyltransferases Ronen Marmorstein 14. Proteomic Identification of the Substrates of Poly-ADP-Ribose Polymerases Yonghao Yu 15. Analysis of the topology of ubiquitin chains Catherine Fenselau 16. Intact protein analysis of troponin proteoforms Ying Ge 17. Assays for tyrosine phosphorylation in human cells Laurie Parker 18. Inhibiting lipid metabolism enzyme activity Ku-Lung (Ken) Hsu 19. Identification and function of N-terminal glyoxylyl modification on proteins Megan Matthews 20. Chromatin immunoprecipitation (ChIP)-Seq analysis of the genomic distribution of histone modifications Michael-Christopher Keogh 21. Identification and characterization of novel protease substrates Christopher Overall 22. Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes David W. Christianson 23. Chemical tools to study redox modifications of cysteine thiols Kate Carroll 24. Quantitative analysis of global protein methylation by mass spectrometry Benjamin A. Garcia 25. Characterization of oxidative protein post-translational modifications Rena AS Robinson 26. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes Jesse Rinehart 27. Preparation of a New Construct of Histone Deacetylase 8 for the Crystallization of Enzyme-Inhibitor Complexes David W. Christianson 28. Analysis of protein deacetylase activity during viral infection Ileana M. Cristea

• ISBN: 978-0-12-818669-5
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/10/2019
• Nº Volúmenes: 1
• Idioma: Inglés