Calorimetry, the latest volume in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Calorimetry is a highly technical experiment and it is easy for new practioners to get fooled into interpreting artifacts as real experimental results. This volume will guide readers to get the most out of their precious biological samples and includes topics on specific protocols for the types of studies being conducted as well as tips to improve the data collection. Most importantly, the chapters will also help to identify pitfalls that need to be avoided to ensure that the highest quality results are obtained. Contains timely contributions from recognized experts in this rapidly changing fieldProvides specific protocols and tips to improve data collection and ensure the highest quality results are obtained Covers research methods in calorimetry, and includes sections on topics such as differential scanning calorimetry of membrane and soluble proteins in detergents. INDICE: 1. Isothermal Titration Calorimetry Measurements of Metal Ions Binding to ProteinsColette F. Quinn ,Margaret C. Carpenter, Molly L. Croteau and Dean E. Wilcox2. Assessing Coupled Protein Folding and Binding through Temperature- Dependent Isothermal Titration CalorimetryDebashish Sahu, Monique Bastidas, Chad W. Lawrence, William G. Noid,and Scott A. Showalter3. Fragment-Based Screening for Enzyme Inhibitors Using CalorimetryMichael I. Recht, Vicki Nienaber and Francisco E. Torres4. Measuring Multivalent Binding Interactions by Isothermal Titration CalorimetryTarun K. Dam, Melanie L Talaga, Ni Fan and Curtis F. Brewer5. Calorimetric and Spectroscopic Analysis of the Thermal Stability of Short Duplex DNA-Containing Sugar and Base-Modified NucleotidesKareem Fakhfakh, Curtis B. Hughesman, A. Louise Creagh, Vincent Kao and Charles Haynes6. Calorimetric Quantification of Cyclodextrin-Mediated Detergent Extraction for Membrane-Protein ReconstitutionMartin Textor and Sandro Keller7. Extending ITC to Kinetics with KinITCPhilippe Dumas, Eric Ennifar, Cyrielle Da Veiga, Guillaume Bec, William Palau, Carmelo Di Primo,Angel Piñeiro, Juan Sabin, Eva Muñoz and Javier Rial8. Measuring the Kinetics of Molecular Association by Isothermal Titration Calorimetry Kirk A. Vander Meulen, Scott Horowitz, Raymond C. Trievel, and Samuel E. Butcher9. Isothermal Titration Calorimetry to Characterize Enzymatic ReactionsLuca Mazzei, Stefano Ciurli and Barbara Zambelli10. Avoiding Buffer Interference in Itc Experiments: A Case Study from the Analysis of Entropy Driven Reactions of Glucose-6-Phosphate DehydrogenaseM. Lucia Bianconi11. ITC Methods for Assessing Buffer/Protein Interactions from the Perturbation of Steady-State Kinetics: A Reactivity Study of Homoprotocatechuate 2,3-DioxygenaseKate L. Henderson, Delta K. Boyles, Vu H. Le, Edwin A. Lewis and Joseph P.Emerson12. Modern Analysis of Protein Folding by Differential Scanning CalorimetryBeatriz Ibarra-Molero, Athi N. Naganathan, Jose M. Sanchez-Ruiz and Victor Muñoz13. A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in DetergentsZhengrong Yang and Christie G. Brouillette14. Orthogonal Methods for Characterizing the Unfolding of Therapeutic Monoclonal Antibodies: Differential Scanning Calorimetry, Isothermal Chemical Denaturation and Intrinsic Fluorescence with Concomitant Static Light ScatteringDeniz B. Temel, Pavel Landsman and Mark L. Brader15. IATC, DSC, and PPC Analysis of Reversible and Multi-State Structural Transition of Cytochrome cShun-ichi Kidokoro and Shigeyoshi Nakamura16. The Complementarity of the Loop to the Stem in DNA Pseudoknots Gives Rise to Local TAT Base-TripletsCalliste Reiling-Steffensmeier and Luis A. Marky17. A High-Throughput Biological Calorimetry Core: Steps to Startup, Run, and Maintain a Multi-User FacilityNeela H. Yennawar, Julia A. Fecko, Scott A. Showalter and Philip C. Bevilacqua
- ISBN: 978-0-12-802906-0
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 11/01/2016
- Nº Volúmenes: 1
- Idioma: Inglés