The use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceContains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease INDICE: 1. Cas9-induced translocations 2. Zinc-finger nuclease design and expression3. Methods and approaches for choosing CRISPR target sites to minimize off-target sites4. Cas9-based genome editing in mice by a single plasmid injection5. In vitro enzymology of Cas96. Determining accuracy of genome editing enzymes (esp. TALENs & Cas9)7. Genome editing in human cells (multiplexing? Orthologous Cas9s?)8. Genome editing with Cas9 nickases9. Genome engineering in human cells10. Developing CRISPR/Cas9 for positive selection genetic screens in vivo.11. Tagging endogenous loci using ZFNs, TALENs, Cas912. Cas9-based transcriptional control in mammalian cells13. Cas9-based genome editing in rats14. Cas9-based genome editing in zebrafish15. Cas9-based genome editing in Xenopus16. Cas9-based genome editing in Drosophila17. Cas9 genome editing in C. elegans18. Transgene-less Cas9 genome editing in C. elegans19. Cas9 genome editing in S. cerevisiae20. Cas9-based genome editing in Arabidopsis and tobacco21. Cas9-based genome editing and gene regulation in bacteria22. Zinc-finger recombinases23. Cas9 for live-cell imaging of genomic loci24. Genome editing for human gene therapy25. Pathway engineering in industrial fungi using Cas926. Nick vs DSB repair

• ISBN: 978-0-12-801185-0
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 514
• Fecha Publicación: 13/12/2014
• Nº Volúmenes: 1
• Idioma: Inglés