CryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their functionContains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted INDICE: 1. Introduction and overview R A. Crowther2. Direct electron detectors Richard Henderson3. Strategies for automated data collection with new detectors Bridget Carragher4. Movie collection and processing John Rubinstein5. Specimen behaviour: ice movement, water molecule movement, radiation damage Robert M. Glaeser6. New grid supports - gold, graphene Christopher J. Russo and Lori A. Passmore 7. RELION Sjors Scheres8. EMAN2 Steve Ludtke9. FREALIGN Nikolaus Grigorieff10. Map validation (tilt pair plots, random phase substitution) and assessment (gold standard overall resolution and local resolution) Peter Rosenthal11. Model building into maps Wah Chiu and Frank DoMaio 12. Refinement of atomic structures Garib Novruz ogly Murshudov13. Segmented processing of helical structures Carsten Sachse14. Sub-tomogram averaging John Briggs15. Microcrystal electron diffraction Tamir Gonen16. Databases and archiving Catherine Lawson and Ardan Patwardhan
- ISBN: 978-0-12-805382-9
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/09/2016
- Nº Volúmenes: 1
- Idioma: Inglés