Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone. Covers the most important laboratory methods in cell biologyChapters written by experts in their fields INDICE: Single Molecule FRET MicroscopyBioluminescent Imaging of Gene ExpressionsConfocal ImagingElectron Microscopy of BoneImmunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular CompartmentsBimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein InteractionsHistochemistryLive ImagingClick Labeling for Single-Molecule FRETHigh Throughput Methods for the Detection of Protein Over Expression Using Fluorescent MarkersCorrelation MicroscopyStereology of the LungFormation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies.Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon MicroscopyCryomicroscopyIn situ HybridizationImaging RNA
- ISBN: 978-0-12-407239-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 464
- Fecha Publicación: 22/02/2013
- Nº Volúmenes: 1
- Idioma: Inglés