Laboratory Methods in Cell Biology: Biochemistry and Cell Culture

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields INDICE: Extraction/Characterization of DNAImmunoprecipitation for Isolation of Protein Signaling Complexes from Native TissuesRealtime PCRSingle Cell Gel ElectrophoresisHigh Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag SystemDifference Gel ElectrophoresisOptimization of Cell Cycle Measurement by Time Lapse-MicroscopyTransfectionCell EnrichmentMagnetic Batch SeparationLateral Flow AssaysMeasurement of Cell RegenerationDNA Damage Analysis in CellsPurification of Cardiac CellsEndothelial Cell Tube Formation in vitro assaysSingle-particle AnalysisMeasurement of Cell InvasivnessTechniques for Cell SortingStem Cells for Drug DiscoveryQuantifying Apoptosis in 96-well PlatesPulse CytometryCell MotilitySingle Cell DepositionEnzyme Sensors for Living CellsMyoblast purifying by magnetic-activated cell sorting (MACS)

• ISBN: 978-0-12-405914-6
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 544
• Fecha Publicación: 14/12/2012
• Nº Volúmenes: 1
• Idioma: Inglés