Cell-Derived Matrices, Part A, Volume 156, provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Specific chapters cover Anisotropic cell-derived matrices with controlled 3D architecture, Generation of functional fluorescently-labelled cell-derived matrices by means of genetically-modified fibroblasts, Bi-layered cell-derived matrices, Engineering clinically-relevant cell-derived matrices using primary fibroblasts, Decellularized matrices for bioprinting applications, and much more. Contains contributions from leading experts in the field from across the globeCovers a wide array of topics on the use of cell-derived matrices for tissue engineering and regenerative medicine applicationsIncludes relevant, analysis-based topics, such as quantification of the mechanical properties, decellularization protocols, and innovative matrix engineering methods INDICE: 1. Anisotropic cell-derived matrices with controlled 3D architecture Josep Samitier Martí Sr. 2. Generation of functional fluorescently-labelled cell-derived matrices by means of genetically modified fibroblasts Daniel Riveline 3. Bi-layered cell-derived matrices Nuno Neves 4. Engineering clinically relevant cell-derived matrices using primary fibroblasts Edna Cukierman 5. Decellularized matrices for bioprinting applications Lorenzo Moroni 6. Cell-derived matrices for tumor cell modelling Virginia Brancato 7. Implementation of a basement membrane invasion assay using mesenteric tissue Armando del Rio Hernandez and Ritobrata Ghose 8. Integration of cell-derived matrices in microfluidic devices Pilnam Kim 9. Characterization of the mechanical properties of CDMs by atomic force microscopy Jordi Alcaraz 10. Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices Robert Linhardt 11. Molecular characterization of cell-derived matrices Barbara Chan 12. A human bone-derived 3D scaffold for studying MSC interactions within the skeletal niche Marietta Herrman 13. Macromolecular crowding in cell culture Dimitrios I. Zeugolis
- ISBN: 978-0-12-820172-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Fecha Publicación: 01/04/2020
- Nº Volúmenes: 1
- Idioma: Inglés