The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, the book provides a detailed background of the enzymatic reaction and the enzyme per se. In addition, a troubleshooting section for each reaction has been added and the layout improved such that the detailed and specific protocols now always feature in a single-page format for convenient use. INDICE: Preface to the Second Edition. Note to the Reader. Abbreviations. 1 Introduction. 2 General Aspects of Enzyme Analysis. 2.1 Basic Requirements for Enzyme Assays. 2.2 What Must Be Observed for an Enzyme Assay? 2.3 Instrumental Aspects. 2.4 Theory of Coupled Enzyme Reactions. 2.5 Substrate Determination. 3 Enzyme Assays. 3.1 Enzyme Nomenclature. 3.2 Practical Considerations forEnzyme Assays. 3.3 Special Enzyme Assays. 3.4 Assays for Enzyme Characterization. 3.5 Enzyme Immunoassays. 4 Binding Measurements. 4.1 Different Types of Binding. 4.2 Binding Measurements by Size Discrimination. 4.3 Spectroscopic Methods. 4.4 Other Binding Methods. 5 Enzymes in Technical Applications. 5.1 Modes of Enzyme Immobilization. 5.2 Methods for Enzyme Immobilization. 5.3 Analysis of Immobilized Enzymes. 5.4 Enzyme Reactors. 5.5 Biosensors. 5.6 ImmobilizedEnzymes in Therapy. Appendix. List of enzymes according to the EC numbers. Index.
- ISBN: 978-3-527-32076-9
- Editorial: Wiley-VCH
- Encuadernacion: Rústica
- Páginas: 376
- Fecha Publicación: 23/02/2011
- Nº Volúmenes: 1
- Idioma: Inglés